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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
 

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

Article DOI: 10.3791/50399-v • 15:23 min • October 1st, 2013
October 1st, 2013 •
1, 1, 1
1Department of Biology, The University of Mississippi

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Microplate based procedures are described for the colorimetric or fluorometric analysis of extracellular enzyme activity. These procedures allow for the rapid assay of such activity in large numbers of environmental samples within a manageable time frame.

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Keywords: Microbial Extracellular Enzyme Activity Nutrient Cycling Carbon Processing Organic Matter Decomposition Nitrogen Mineralization Phosphorus Mineralization Colorimetric Assay Fluorometric Assay High-throughput Microplate Ecosystem Processes
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