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Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
 

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Article DOI: 10.3791/50448-v • 11:40 min • June 25th, 2013
June 25th, 2013 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

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Resumo
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The yeast mitochondrial nucleoid protein, Mgm101, is a Rad52-type recombination protein that forms large oligomeric rings. A protocol is described to prepare soluble recombinant Mgm101 using the Maltose Binding Protein (MBP)-tagging strategy coupled with cation exchange and size exclusion chromatography.

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Keywords: Mgm101 Recombinant Protein MBP-tagged E. Coli Expression Affinity Chromatography Cationic Exchange Chromatography Size Exclusion Chromatography Mitochondrial DNA Repair Rad52-type Recombination Proteins
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