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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
 

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

Article DOI: 10.3791/50456-v • 17:01 min • July 18th, 2013
July 18th, 2013 •
1, 1
1Department of Chemical Engineering, Massachusetts Institute of Technology

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We present a simple protocol to obtain fluorescence microscopy movies of growing yeast cells, and a GUI-based software package to extract single-cell time series data. The analysis includes automated lineage and division time assignment integrated with visual inspection and manual curation of tracked data.

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Keywords: Fluorescence Time-lapse Microscopy Single-cell Dynamics Gene Expression Budding Yeast GRAFTS MATLAB Microfluidic Culture Device Cell Segmentation Cell Tracking Lineage Analysis Time Series Analysis
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