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JoVE Journal Neuroscience
Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities
 

Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

Article DOI: 10.3791/50557-v • 08:10 min • March 31st, 2014
March 31st, 2014 •
1, 1, 1, 2, 1
1Department of Molecular Physiology & Biophysics, University of Iowa Carver College of Medicine, 2Department of Biology & Biochemistry, University of Bath

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We describe the use of styryl FM dyes to image synaptic vesicle recycling in functional nerve terminals. This protocol can be applied not only to evoked, but also spontaneous and miniature synaptic activities. The protocol expands the variety of synaptic events that can be effectively evaluated.

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Synaptic Vesicle Recycling FM Dyes Evoked Synaptic Activity Spontaneous Synaptic Activity Miniature Synaptic Events Styryl FM Dyes Membrane Turnover Conventional Protocols Weaker Synaptic Activities Imaging System Sensitivity Photobleaching Of FM Dyes
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