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Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
 

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Article DOI: 10.3791/50558-v • 08:23 min • September 1st, 2013
September 1st, 2013 •
1,2, 1, 1, 1,3, 1
1Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health, 2Department of Biology, University of North Carolina at Chapel Hill, 3Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering, Rutgers University

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Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. In this article, we present a detailed method for imaging the dynamics of subcellular structures, such as the secretory granules, in the salivary glands of live mice.

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Intravital Microscopy Live Mice Imaging Subcellular Structures Fluorescent Proteins Confocal Microscopy Salivary Glands Motion Artifacts Spatial Resolution Confocal Microscopy Two-photon Microscopy Pharmacological Manipulation Genetic Manipulation Exocytosis Secretory Granules Acinar Cells Apical Plasma Membrane Beta-adrenergic Receptors Transgenic Mouse Cytosolic GFP Tandem-Tomato
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