Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Implanting Glass Spinal Cord Windows in Adult…
JoVE Journal
Medicine

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicine
Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis
 

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Article DOI: 10.3791/50826-v • 09:20 min • December 21st, 2013
December 21st, 2013 •
1, 1, 1,2, 1,2
1Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, 2Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

Capítulos

Resumo
Automatic Translation

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

Tags

Keywords: Intravital Microscopy Spinal Cord Experimental Autoimmune Encephalomyelitis (EAE) Glass Window Implantation Thy1-CFP-23 Mice Axonal Loss Cell Dynamics Autoimmune Demyelinating Diseases
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter