Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Submillisecond Conformational Changes in Proteins …
JoVE Journal
Chemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Chemistry
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
 

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Article DOI: 10.3791/50969-v • 10:02 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Chemistry and Biochemistry, Florida International University

Capítulos

Resumo
Automatic Translation

Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

Tags

Keywords: Photothermal Beam Deflection Photo-acoustic Calorimetry Thermal Grating Conformational Changes Proteins Microsecond To Millisecond Time-scales Volume Changes Enthalpy Changes Calcium Binding Calcium Transducers Neuronal Calcium Sensors DM-nitrophen Photo-triggering Fast Kinetics
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter