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Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
DOI:

11:31 min

August 28, 2014

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Capítulos

  • 00:05Título
  • 01:41Activating the Surface of Glass Coverslips
  • 03:42Preparation of Hydroxy-PAAm Hydrogels
  • 05:50Micropatterning Hydroxy-PAAm Hydrogels
  • 09:09Results: Micropatterned Hydroxy-PAAm Hydrogels Enable Independent Tuning of Mechanotransduction Cues In Vitro
  • 10:55Conclusion

Summary

Tadução automática

We present a new polyacrylamide hydrogel, called hydroxy-PAAm, that allows a direct binding of ECM proteins with minimal cost or expertise. The combination of hydroxy-PAAm hydrogels with microcontact printing facilitates independent control of many cues of the natural cell microenvironment for studying cellular mechanostransduction.

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