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JoVE Journal Bioengineering
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
 

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Article DOI: 10.3791/51010-v • 11:31 min • August 28th, 2014
August 28th, 2014 •
1, 1, 1
1Laboratoire Interfaces et Fluides Complexes, Université de Mons

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Resumo
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We present a new polyacrylamide hydrogel, called hydroxy-PAAm, that allows a direct binding of ECM proteins with minimal cost or expertise. The combination of hydroxy-PAAm hydrogels with microcontact printing facilitates independent control of many cues of the natural cell microenvironment for studying cellular mechanostransduction.

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Keywords: Hydroxy-PAAm Hydrogels Polyacrylamide Hydrogels Mechanotransduction Extracellular Matrix (ECM) Cell-ECM Interactions Cell Morphology Matrix Stiffness ECM Protein Immobilization Microcontact Printing Endothelial Cells Nucleus Mechanics
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