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JoVE Journal Bioengineering
Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
 

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

Article DOI: 10.3791/61671-v • 07:41 min • December 4th, 2020
December 4th, 2020 •
1, 2, 3, 3, 2, 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

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The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain regimes.

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Controlled Strain 3D Hydrogels Live Microscopy Imaging Biomechanical Responses Hydrogel Stretching Live Imaging Hydrogel Geometry Manipulation Cells And Extracellular Matrix Forces Tissue Development Diseases Progress Cancer Therapies Tissue Engineering Models Laser Cutter Rubber Strip Hydrophobic Film Plastic Dish Sealing Film Silicone Strips Fibrin Gel Formation Labeled Fibrinogen Cooled Thrombin
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