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JoVE Journal Bioengineering
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
 

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Article DOI: 10.3791/60944-v • 06:53 min • May 17th, 2020
May 17th, 2020 •
1, 1, 2,3, *1,3,4,5, *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally

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Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.

Tags

Custom Multiphoton Microscopy Platform Live Imaging Mouse Cornea Conjunctiva Dual Fluorescent Transgenic Mouse Model Ocular Surface Multiphoton Microscope Water Immersion 20X Objective Titanium Sapphire Laser Excitation Source EGFP TdTomato Dichroic Mirrors SHG EGFP TdTomato Signals Eye Holder Anesthetized Mouse Heated Microscope Stage Temperature Monitoring Probe Stereotaxic Mouse Holder External Auditory Meatus Oxybuprocaine Hydrochloride Solution Saline Solution
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