Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Visualization of the Immunological Synapse by…
JoVE Journal
Immunology and Infection

Este de .

JoVE Journal Immunology and Infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
 

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Article DOI: 10.3791/51100-v • 10:00 min • March 24th, 2014
March 24th, 2014 •
1, 1
1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

Capítulos

Resumo
Automatic Translation

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

Tags

Keywords: Immunological Synapse Natural Killer Cells Actin Reorganization Super-resolution Imaging Stimulated Emission Depletion (STED) Nanoscopy Time-gated STED Cytotoxic Synapse F-actin Architecture
Read Article
Waiting X
Simple Hit Counter