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Immunology and Infection

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JoVE Journal Immunology and Infection
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
 

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Article DOI: 10.3791/51112-v • 12:18 min • November 6th, 2013
November 6th, 2013 •
1,2, 1,3, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

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Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface proteins.

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Keywords: Baculovirus Expression System Recombinant Hemagglutinin Recombinant Neuraminidase H7N9 Influenza Virus Glycoproteins Immunological Assays Vaccine Standardization Antibody Discovery NA Enzymatic Function Antivirals Recombinant Vaccine
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