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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
 

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

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Protein-DNA Interaction Optical Tweezers Single-molecule Fluorescence Lactose Repressor DNA Target Sequence 1D Diffusion Molecular Motors Single-molecule Manipulation Single-molecule Imaging
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