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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
 

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Article DOI: 10.3791/51709-v • 10:24 min • August 29th, 2014
August 29th, 2014 •
1, 1
1Energy, Mining and Environment, National Research Council Canada

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Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.

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Next-generation Sequencing 16S Ribosomal RNA Gene Amplicons Microbial Ecology Sequencing Depth Benchtop Sequencers PCR Amplification Cloning Sanger Sequencing Next-generation Sequencer Environmental DNA Primers Sequencing Adapters Barcodes Emulsion PCR Disposable Chip Fastq Format Sample Membership Taxonomy-finding Algorithm Mothur Software
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