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Immunology and Infection

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JoVE Journal Immunology and Infection
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
 

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Article DOI: 10.3791/51806-v • 07:54 min • August 19th, 2014
August 19th, 2014 •
1, 1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

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DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.

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Chromosome Aberrations Mouse B Lymphocytes PNA-FISH DNA Repair Genomic Instability Tumorigenesis Metaphase Chromosome Spreads Ploidy Mitotic Index Primary Cell Population Transformed Fibroblasts Patient Cell Lines
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