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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
 

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

Article DOI: 10.3791/63987-v • 08:20 min • August 17th, 2022
August 17th, 2022 •
*1, *1,2, 1, 1
1Instituto René Rachou, Fundação Oswaldo Cruz, 2Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais
* These authors contributed equally

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Resumo
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Here we describe a new method to help elucidate the mechanisms of cellular immunity to Plasmodium during the blood stage of infection. This is an in vitro assay that measures infected red blood cell killing by cytotoxic lymphocytes. 

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In Vitro Assay Plasmodium-infected Red Blood Cell Killing Cytotoxic Lymphocytes Cell Immunity Blood Stage Malaria Therapeutic Targets Malaria Vaccine Progress Malaria Pathogenesis Protective Malaria Immunity Mechanisms Of Cell Immunity Luna De Lacerda Christopher Gomes Procedure Demonstration Mouse Blood Extraction Mouse Spleen Removal Effector Cell Population Purification
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