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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
 

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Article DOI: 10.3791/51906-v • 12:35 min • July 6th, 2014
July 6th, 2014 •
1, 1, 1, 1,2
1Department of Medical Microbiology, University of Manitoba, 2Department of Community Health Sciences, University of Manitoba

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The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

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Keywords: Mucosal Immunity Female Genital Tract HIV Transmission Sexually Transmitted Infections Cervical Mononuclear Cells Flow Cytometry Cervical-vaginal Lavage Immune Mediators
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