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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
 

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Resumo
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Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.

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Keywords: Enterobacter Sp. YSU Auxotrophs Transposon Mutagenesis Tn5 Transposome M-9 Minimal Medium R6K Gamma Replication Origin Kanamycin Resistance Sanger Sequencing BLAST Gene Identification
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