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Recovery of Adult Zebrafish Hearts for High-throughput Applications
 

Recovery of Adult Zebrafish Hearts for High-throughput Applications

Article DOI: 10.3791/52248-v • 06:51 min • December 12th, 2014
December 12th, 2014 •
1, 2,3, 2,3
1Cardiovascular Research Institute and Division of Cardiology, University of California San Francisco, 2Department for Biochemistry and Biophysics, University of California San Francisco, 3Max-Planck Institute for Heart and Lung Research

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Use of zebrafish for cardiovascular research is expanding towards research on adult hearts. For these applications, quick and simple isolation of cardiac tissues is key to avoid post-mortem changes and to obtain an adequate number of samples. Here, we describe a fast and reproducible method for dissecting adult zebrafish hearts.

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Zebrafish Adult Hearts High-throughput Applications Cell Dissociation RNA Purification DNA Purification Protein Purification Gene Regulatory Changes Metabolic Changes Heart Structure Heart Regeneration Zebrafish Heart Dissection Specialized Tools Large-scale Dissection Heart Architecture Painless Procedure Fresh Specimens Fixed Specimens Cardiovascular Research
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