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JoVE Journal Developmental Biology
Måling Protein Stabilitet i Living zebrafisk embryoner anvendelse af fluorescens Decay Efter fotoomdannelse (FDAP)
 
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Måling Protein Stabilitet i Living zebrafisk embryoner anvendelse af fluorescens Decay Efter fotoomdannelse (FDAP)

Article DOI: 10.3791/52266-v • 09:45 min • January 28th, 2015
January 28th, 2015 •
1, 2, 1, 2
1Department of Molecular and Cellular Biology, Harvard University, 2Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

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Protein niveauer i celler og væv er ofte stramt reguleret af balance proteinproduktion og clearance. Anvendelse af fluorescens Decay Efter fotoomdannelse (FDAP) kan clearance kinetik proteiner eksperimentelt målt in vivo.

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Developmental Biology Fluorescens Decay Efter fotoomdannelse (FDAP) protein stabilitet clearance rate konfokal mikroskopi photoconvertible protein Dendra2 embryo zebrafisk
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