JoVE Journal
Bioengineering
Bioengineering
This Conteúdo is Open Access.
Capítulos
Resumo
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.