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A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry
 

A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry

Article DOI: 10.3791/52455-v • 09:54 min • March 11th, 2015
March 11th, 2015 •
1, 1, 1, 1, 1
1Department of Biological Sciences, University of Alberta

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The goal of this procedure is to demonstrate the reproducibility and adaptability of using a microtiter plate format for microalgal screening. This rapid screen combines WATER-Pulse-Amplitude-Modulated (WATER-PAM) fluorometry to measure photosynthetic yield as an indicator of Photosystem II (PSII) health with small volume bacterial-algal co-cultures.

Tags

Small Volume Bioassay Bacterial/phytoplankton Co-culture WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry Microalgae Manipulation Culture Subsampling Pseudo-replication Axenic Cultures Microtiter Plates Experimental Volume Extensive Replication Bacterial-algal Co-cultures Algal Physiology Tests Toxin Screening
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