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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
 

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Article DOI: 10.3791/52589-v • 05:58 min • April 20th, 2016
April 20th, 2016 •
1,2, 1, 1,2,3, 1,3
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, 2Boston Children's Hospital, Division of Gastroenterology, Harvard Medical School, 3Humanitas Clinical and Research Center

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Resumo
Automatic Translation

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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Keywords: Single-cell Suspension Cytofluorimetric Analysis Mouse Skin Resident Immune Cells Skin Sampling Skin Digestion Skin Tissue Preparation Skin Cell Labeling
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