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Immunology and Infection

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JoVE Journal Immunology and Infection
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
 

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Article DOI: 10.3791/52655-v • 13:36 min • May 6th, 2015
May 6th, 2015 •
1, 1, 1, 1,2
1Department of Medicine, University of Alabama at Birmingham, 2Center for AIDS Research, University of Alabama at Birmingham

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Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.

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Keywords: Adenovirus Serotype 5 Ad5 Antigen Capsid-incorporation Strategy Vaccine Development HIV Epitope His6 Tag Hexon Hypervariable Region Neutralization Assay Humoral Immunity Rare-serotype Adenovirus
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