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Immunology and Infection

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JoVE Journal Immunology and Infection
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
 

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistic transformation is a method used to generate stable integration of DNA into the genome of the opportunistic pathogen Cryptococcus neoformans through homologous recombination. We will demonstrate biolistic transformation of a construct, which has the gene encoding acetate kinase fused to the fluorescent tag mCherry into C. neoformans.

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Keywords: Biolistic Transformation Fluorescent Tagged Gene Cryptococcus Neoformans Opportunistic Fungal Pathogen Acetate Production Xylulose-5-phosphate/fructose-6-phosphate Phosphoketolase Acetate Kinase MCherry Homologous Recombination Fungal Meningitis
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