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JoVE Journal Developmental Biology
Immunostaining to Visualize Murine Enteric Nervous System Development
 

Immunostaining to Visualize Murine Enteric Nervous System Development

Article DOI: 10.3791/52716-v • 07:54 min • April 29th, 2015
April 29th, 2015 •
1, 1, 2, 1,2,3
1Department of Surgery, University of Wisconsin School of Medicine and Public Health, 2Department of Neurosciences, University of Wisconsin School of Medicine and Public Health, 3Department of Pediatrics, University of Wisconsin School of Medicine and Public Health

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Resumo
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The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.

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Keywords: Enteric Nervous System Neural Crest Cells Cholinergic Neurons Choline Acetyltransferase (ChAT) Immunostaining Embryonic Development Gastrointestinal Tract Neurotransmitter Expression
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