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JoVE Journal Developmental Biology
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
 

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Article DOI: 10.3791/59113-v • 10:03 min • May 10th, 2019
May 10th, 2019 •
*1,2, *2, 2
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan
* These authors contributed equally

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Resumo
Automatic Translation

Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

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Tissue Preparation Immunostaining Mouse Craniofacial Tissues Undecalcified Bone Sectioned Materials Antigen Retrieval Decalcification Immunostaining Results Time-saving Method Labor-saving Method Visual Demonstration Target Tissue 10% EDTA Treatment Cryoprotection PBS Dishes Culture Plate Pregnant Mouse Embryo Dissection
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