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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
 

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Article DOI: 10.3791/52753-v • 09:07 min • May 27th, 2015
May 27th, 2015 •
1, 1, 1, 1, 1,2, 1
1Department of Pathology, Stanford University School of Medicine, 2Department of Microbiology & Immunology, Stanford University School of Medicine

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We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.

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Keywords: Mast Cells In Vivo Knock-in Mice Bone Marrow-derived Cultured Mast Cells Adoptive Transfer Anatomical Distribution Host Defense Allergic Reactions
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