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Journal / Developmental Biology / Isolation and Characterization of Satellite Cells …
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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
 

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Article DOI: 10.3791/52802-v • 07:37 min • July 20th, 2015
July 20th, 2015 •
1, 2, 3, 1, 1, 1
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

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Keywords: Satellite Cells Head Branchiomeric Muscles Fibrosis Muscle Regeneration Cleft Palate In Vitro Culture Tissue Engineering Enzymatic Digestion Extracellular Matrix Gel
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