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Journal / Developmental Biology / Isolation and Characterization of Cardiac…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
 

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Article DOI: 10.3791/57263-v • 09:16 min • February 28th, 2018
February 28th, 2018 •
*1,2, *1, 1, 1, 3, 3, 3, 2,3, 1,2, *3, *1
1Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health, Università degli Studi di Milano, 3Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS
* These authors contributed equally

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Resumo
Automatic Translation

In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.

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Keywords: Cardiac Mesenchymal Stromal Cells Arrhythmogenic Cardiomyopathy Endomyocardial Biopsy Adipogenic Differentiation Phenotyping Cell Isolation Cell Culture Collagenase Digestion Surface Markers
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