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In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
 

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Article DOI: 10.3791/53302-v • 09:33 min • January 5th, 2016
January 5th, 2016 •
1
1Department of Biological Sciences, St John's University

Capítulos

Resumo
Automatic Translation

The present report describes an in vitro enzymatic assay to measure phosphatidylethanolamine methyltransferase activity using Leishmania cell extracts. This assay is based on the transfer of a radioactive methyl group from S-[Methyl-3H]adenosyl-L-methionine onto endogenous phosphatidylethanolamine.

Tags

Keywords: Phosphatidylethanolamine Methyltransferase PEMT Activity Lipid Metabolism Phosphatidylcholine Biosynthesis Whole Cell Extract Leishmania Cells Cell Culture Cell Lysis Protein Quantification BCA Assay
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