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Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
 

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

Article DOI: 10.3791/53329-v • 08:56 min • January 29th, 2016
January 29th, 2016 •
1, 2, 2, 1
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 2FirstString Research, Inc.

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This protocol describes how to use quantitative Real-time PCR (QRT-PCR) to detect tumor cell specific mRNA representing metastasis within the mouse lung tissue.

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Keywords: Lung Metastasis Mouse Mammary Tumor Models Quantitative Real-time PCR RNA Isolation Tissue Homogenization Lysis Buffer Proteinase K RNA Precipitation RNA Column Purification
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