Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Examination of Proteins Bound to Nascent DNA in…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
 

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Article DOI: 10.3791/53647-v • 09:14 min • January 14th, 2016
January 14th, 2016 •
1
1Department of Radiation Oncology, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine

Capítulos

Resumo
Automatic Translation

In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.

Tags

Keywords: BrdU-ChIP-Slot-Western Nascent DNA Mammalian Cells DNA Replication DNA Repair Chromatin Formaldehyde Crosslinking Sonication Protein A Agarose Immunoprecipitation Input Control
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter