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JoVE Journal Bioengineering
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
 

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Article DOI: 10.3791/54102-v • 07:37 min • June 26th, 2016
June 26th, 2016 •
1, 2, 2, 2, 3, 4
1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

Capítulos

Resumo
Automatic Translation

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

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Keywords: Circulating MicroRNA Droplet Digital PCR DNA-binding Dye Chemistry Biomarker Cancer Diagnosis Plasma Serum Reverse Transcription DdPCR Mix CDNA Template Droplet Generation
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