JoVE Journal
Immunology and Infection
Immunology and Infection
This Conteúdo is Open Access.
Capítulos
Resumo
We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.