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Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
 

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Article DOI: 10.3791/54257-v • 09:48 min • July 27th, 2016
July 27th, 2016 •
1, 1, 1, 1, 1, 1, 1, 2, 1, 1
1Division of Gastroenterology, Hepatology and Nutrition, Virginia Commonwealth University, 2McGuire Veterans Affairs Medical Center, Research BC-101

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MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.

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Keywords: MiRNA Isolation High-throughput High Density Lipoprotein HDL Plasma Exosome Precipitation Density Gradient Ultracentrifugation VLDL LDL Cardiology Hepatology
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