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Cancer Research

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JoVE Journal Cancer Research
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
 

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

Article DOI: 10.3791/54270-v • 08:00 min • October 14th, 2016
October 14th, 2016 •
1, 1, 1,2
1Institut pour la Recherche sur le Cancer de Lille (IRCL), INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

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Resumo
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This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.

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Keywords: C1498 Cells Murine Myelomonocytic Leukemia Acute Leukemia Animal Model Congenic C57 Black 6 Mouse Tail Vein Injection Orbital Sinus Blood Draw Mononuclear Cell Isolation Flow Cytometry Bone Marrow Isolation Leukemia Development Immune Deficiency Chemo And Immunotherapeutic Agents
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