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Biochemistry

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JoVE Journal Biochemistry
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
 

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Article DOI: 10.3791/54463-v • 09:09 min • September 20th, 2016
September 20th, 2016 •
1,2, 1,2, 3
1The Bridge Institute, University of Southern California, 2Department of Chemistry, University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute, Arizona State University

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Resumo
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We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

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Keywords: Protein Microcrystals Lipidic Cubic Phase Serial Femtosecond Crystallography Membrane Protein Structure Protein Reconstitution Sample Preparation Syringe Handling Precipitant Solution Protein-laden LCP Teflon Spherules Sample Sealing
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