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Immunology and Infection

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JoVE Journal Immunology and Infection
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
 

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Article DOI: 10.3791/54599-v • 07:14 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 3, 2, 2, 3, 3, 3, 1, 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

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This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information obtained in the experimental model.

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Phenotypic Characterization Macrophages Rat Kidney Flow Cytometry Resident Macrophages Quantification Protein Marker Kidney Injury Cellular Integrity Kidney Perfusion Sedation Surgical Table Abdominal Skin Peritoneum Pleural And Abdominal Cavities Perfusion System Saline Solution Renal Hilum Decapsulation H.B.S.S. Kidney Cell Suspension
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