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JoVE Journal Biochemistry
Defining Substrate Specificities for Lipase and Phospholipase Candidates
 

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Article DOI: 10.3791/54613-v • 08:59 min • November 23rd, 2016
November 23rd, 2016 •
1, 1, 1, 1, 1
1Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México

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Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.

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Keywords: Lipase Phospholipase Substrate Specificity Lipid Remodeling Cell-free Protein Extract Enzyme Assay Para-nitrophenol Radiolabeling Acetate
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