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ライブ細胞懸濁液の蛍光光度アッセイでFRETセンサーを用いて測定したGタンパク質選択的なGPCRの立体配座

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ライブ細胞懸濁液の蛍光光度アッセイでFRETセンサーを用いて測定したGタンパク質選択的なGPCRの立体配座

Article DOI: 10.3791/54696-v • 09:12 min • September 10th, 2016

September 10th, 2016 •

Ansley Semack¹, Rabia U. Malik², Sivaraj Sivaramakrishnan¹

¹Genetics, Cell Biology, and Development, University of Minnesota, ²Department of Cell and Developmental Biology, University of Michigan

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分子生物学、問題115、GPCRシグナル伝達、FRET、HEK-293、生細胞、ER / K SPASMリンカ、mCerulean、mCitrine、アゴニスト主導のシグナリング

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