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Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
 

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Article DOI: 10.3791/54725-v • 08:00 min • December 4th, 2016
December 4th, 2016 •
1, 2, 1, 2, 1
1Southern California Coastal Water Research Project Authority, 2Department of Physiological Sciences, University of Florida

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A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

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Keywords: Endocrine Activity Water Quality In Vitro Transactivation Bioassays Receptor-based Cell Assays Endocrine Disrupting Chemicals Water Sample Preparation Dilution Series Solvent Control Cell Thawing Cell Culture
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