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JoVE Journal Biochemistry
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
 

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Article DOI: 10.3791/54971-v • 08:53 min • January 11th, 2017
January 11th, 2017 •
1, 1
1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology, Case Western Reserve University School of Medicine

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Resumo
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This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

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Keywords: Scaffold Liposomes Lipid-proximal Protein-protein Interactions Mitochondrial Dynamics Protein Complexes Membrane Compartments Lipid Templates Native Confirmation Lipid Cofactors Mitochondrial Fission Machinery His-tagged Proteins Mff Buffer A BME Lipid Extrusion
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