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Immunology and Infection

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JoVE Journal Immunology and Infection
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
 

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetic factors can interact with genetic programs to modulate gene expression and regulate B cell function. By combining in vitro B-cell stimulation, qRT-PCR, and high-throughput microRNA-sequence and mRNA-sequence approaches, we can analyze the epigenetic modulation of miRNA and gene expression in B cells.

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Genome-wide Analysis HDAC Inhibitor MicroRNA MRNA B Cells Class-switch DNA Recombination Plasma Cell Differentiation Epigenetic Regulation Antibody Response Immunoglobulin Class-switching Pathogen-free C57BL6J Mouse Spleen Extraction Complete RPMI 1640 Medium Cell Strainer Polypropylene Centrifuge Tube
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