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Journal / Immunology and Infection / Bリンパ芽球様細胞株の樹立のためのシンプルな赤血球溶解法…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bリンパ芽球様細胞株の樹立のためのシンプルな赤血球溶解法
 
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Bリンパ芽球様細胞株の樹立のためのシンプルな赤血球溶解法

Article DOI: 10.3791/55191-v • 09:49 min • January 14th, 2017
January 14th, 2017 •
1,2, 2, 2
1Wuhan Institute of Virology, Chinese Academy of Sciences, 2Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

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B-LCLを確立するための単純な赤血球溶解方法は、少量の血液を必要とし、凍結保存し、開始からの時間を節約し、高い不死化効率で開発されました。

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免疫号119、EBV、赤血球溶解法、形質転換、B-LCLを、PBMC、密度勾配分離法
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