Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Organotypic Hippocampal Slice Cultures As a Model …
JoVE Journal
Neuroscience
Author Produced

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Neuroscience
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
 

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Article DOI: 10.3791/55359-v • 07:49 min • August 27th, 2017
August 27th, 2017 •
*1, *1, 2, 1
1Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 2Department of Anatomy, University of Otago
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.

Tags

Organotypic Hippocampal Slice Cultures Neuroprotection Tumor Cells Slicing Procedure Vibratome Agar Blocks Autoclave Set Contamination Cyanoacrylate Glue Petri Dish Preparation Medium Specimen Holder Mechanical Stability Vibratome Tissue Dissection OHSC (Organotypic Hippocampal Slice Culture) Pasteur Pipette Binocular Microscope Hippocampal Region
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter