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Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
 

Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation

Article DOI: 10.3791/64234-v • 07:16 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 2, 1
1Laboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center, Lund University, 2Lund Stem Cell Center, Lund University

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This protocol describes long-term organotypic cultures of adult human cortex combined with ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors, which present a novel methodology to further test stem cell-based therapies for human neurodegenerative disorders.

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Keywords: Organotypic Cultures Adult Human Cortex Ex Vivo Model Human Stem Cell Transplantation Neural Circuitry Brain Damage Tissue Slices Vibratome Cutting Solution Tissue Adhesive Coronal Slices Sagittal Slices Rinsing Solution
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