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JoVE Journal Neuroscience
Analyzing Dendritic Morphology in Columns and Layers
 

Analyzing Dendritic Morphology in Columns and Layers

Article DOI: 10.3791/55410-v • 08:41 min • March 23rd, 2017
March 23rd, 2017 •
1, 2, 3, 3, 3, 1
1Section on Neuronal Connectivity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), 2Mathematical and Statistical Computing Laboratory, Center for Information Technology, National Institutes of Health (NIH), 3Biomedical Imaging Research Services Section, Center for Information Technology, National Institutes of Health (NIH)

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Here, we show how to analyze dendritic routing of Drosophila medulla neurons in columns and layers. The workflow includes a dual-view imaging technique to improve the image quality and computational tools for tracing, registering dendritic arbors to the reference column array and for analyzing the dendritic structures in 3D space.

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Keywords: Dendritic Morphology Drosophila Medulla Neurons Columns Layers Neuroscience Dendritic Wiring Imaging Confocal Microscopy GFP Labeling Brain Clearing Sample Preparation Tissue Orientation
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