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Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
 

Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

Article DOI: 10.3791/55495-v • 08:05 min • July 3rd, 2017
July 3rd, 2017 •
*1,2,3, *2, 2, 2, 1, 2,4,5
1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), Singapore National Eye Center, 3School of Material Science and Engineering, Nanyang Technological University, 4Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, 5Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School
* These authors contributed equally

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Resumo
Automatic Translation

Live-cell imaging of the labeled blood cells in ocular circulation can provide information about inflammation and ischemia in diabetic retinopathy and age-related macular degeneration. A protocol to label blood cells and image the labeled cells in the retinal circulation is described.

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Keywords: Erythrocytes Leukocytes Retinal Circulation Mouse Fluorescent Dye Labeling ICG Sodium Fluorescein Scanning Laser Ophthalmoscope Blood Cell Flow Dynamics Retinal Diseases
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